NUCLEIC ACID DETECTION METHOD

The present invention constitutes a most advantageous alternative to methods of the state of the art. The method of the present invention works more efficiently than existing methods, results in an authentic exponential chain reaction; offers a reduction of background signals whencompared to them; and provides a tool to detect both RNA and DNA. The method of the invention allows the detection of nucleic acid amounts as low as 100 fM with a high signal-to-noise ratio and without the need to perform any amplification of the nucleic acid of interest. The present invention also relates to a kit to carry out the method for detecting the presence of a nucleic acid sequence in a sample, and to the use of the method or the kit of the invention for the diagnosis or prognosis of a disease or condition.