DEVICE FOR MEASURING INTRINSIC AUTOFLUORESCENCE OF A BIOLOGICAL SAMPLE AND METHOD USING THEREOF

The present invention refers to a device for measuring intrinsic autofluorescence of molecules present in a biological sample wherein said device comprises: a) a light-sheet, virtual light-sheet or light line delivery pathway module comprising a laser generator capable of generating ultrashort pulses with a duration between 1 microsecond and 1 attosecond, and optical elements shaping the laser light into a light sheet, virtual light-sheet or light-line; b) an imaging chamber comprising an imaging dish inside of which said biological sample is placed, a translational stage, an illumination objective and a collection objective both immersed in an immersion liquid in a sealed immersion chamber, wherein both objectives are positioned below the imaging dish, wherein said immersion chamber is placed inside an incubation chamber, allowing environmental control; and c) a fluorescence detection pathway comprising optical elements, light-splitting elements such as diffractive elements or dichroic mirrors, an array detector, and a device control and signal processing unit, wherein said fluorescence detection pathway has means for collecting the spectrum of fluorescence of said biological sample building a multi or hyper spectral data cube. In a second aspect, the present invention relates to a method for assessing quality of a biological sample by measuring intrinsic autofluorescence of molecules present in said biological sample, using the device described above.