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NANOSTRUCTURED ELECTRODES FOR THE ELECTRICAL STIMULATION OF CULTURED CELLS, AND DEVICES, SYSTEMS AND PROCEDURES ASSOCIATED THEREWITH
PCT/ES2021/070462; 202030626
The present invention relates to an electrical cell stimulation electrode (1), adapted for the insertion thereof into a miniaturised cell culture mini-well (3) plate (2), comprising at least one material disposed as a substrate (4) and an electrically conductive material disposed on said substrate (4). Advantageously, the electrically conductive material is formed by metallic nanopillars (5), deposited on the substrate (4) by means of glancing angle deposition (GLAD) with magnetron cathode sputtering. The invention likewise relates to different devices, systems, uses and procedures associated with said electrodes (1).
The potential outcome of this research leads to creation of therapeutic implantable devices that deliver efficient and optimized dosage of electrical signals that is manageable by patients and/or the doctors, to improve and accelerate healing of defected tissues. The innovative and well-characterized system, provides a standard platform for applying electrical simulation to the cells in culture and assess the changes using in situ imaging. This is important since most of voltage and calcium reporters are detected by means of live-imaging. Therefore, the possibility of in situ imaging is a critical feature in such devices.