HANDLING OF MEMBRANE-SHAPED TISSUE

CELOX is a toolkit for fixation and handling of membrane-shaped tissue in biomedical tissue engineering and research. It facilitates working with biological membranes such as amniotic tissue, RHC collagen membrane, extremely fragile CLP collagen membrane and synthetic PDLLA. It is designed for culturing in standardmulti well plates. The processing time in the lab is dramatically shorter compared to the traditional inserts. CELOX has been developed to facilitate and standardize the handling and fixation of membrane tissue. The complete set consists of a platform fitting in a petri dish, a cutting layer, 3 gripping tools of different size and a series of flexible and hard rings to fit in 6, 12 or 24-well plates. The processing time and the risk of damage is minimised by reducing the number of manipulations. The consumption of biological membrane is considerably lower due to the visual overview and easy distribution over the 3 ring sizes. Once the membrane is fixed on the ring, it can be easily manipulated, either directly with the gripping tool or with tweezers. The ring fits in multi-well plates of the desired diameter. The membrane can be placed on the bottom of the well plate, or at a distance, by means of the feet on the rubber ring. It can be completely submerged in one culture medium, or provided with different media above and below.​