METHOD OF EXTRACTION OF HIGH QUALITY DNA IN BIVALVE MOLLUSC TEXTILES.

Advantages / Differential Points Non‑toxic reagents and safer handling: Buffers used do not contain toxic components (e.g., avoid β‑mercaptoethanol, phenol, chloroform) and can be handled without additional safety measures; no DNA‑binding resins required. Speed: Reduces extraction time from ~2 days to ~5 hours for bivalve tissues; among the fastest methods referenced by the applicants. [2332757 | PDF] Cost‑effective and accessible: Low buffer cost, no special equipment; buffers can be prepared quickly in‑house, suitable for labs with limited budgets and even non‑specialized personnel. Broad tissue and sample compatibility: Works on mantle, muscle, gonad, and gill; effective for fresh, frozen, ethanol‑preserved, and manufactured (canned) samples; in canned products, DNA can be used directly for PCR. High‑quality output and stability: Yields high‑molecular‑weight genomic DNA; DNA is stable at 4 °C (up to ~2 years) and at −20 °C (more than 3 years). Defined, simple protocol: Lysis (~20 mg tissue) at 65 °C for ~1.5 h, ammonium acetate (5 M) precipitation, isopropanol/ethanol precipitation, wash and resuspension; proteinase K step optional. Applications Food authenticity and labeling compliance for fishery and aquaculture products analyzed by food testing laboratories. Basic research applications requiring PCR‑grade genomic DNA from bivalve tissues.