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METHOD OF EXTRACTION OF HIGH QUALITY DNA IN BIVALVE MOLLUSC TEXTILES.
ES2332757
Method of extraction of high quality dna in tissues of bivalve molluscs. The isolation and purification of dna is the prerequisite for the realization of molecular techniques. Despite the numerous commercial kits and protocols existing in the literature, there is no specific dna extraction method for mollusc tissues. The present method allows the rapid and economic purification of genomic dna from different tissues of bivalve molluscs: mantle, muscle, gonad and gill. The present methodology allows the extraction of dna from fresh products, frozen, preserved in ethanol and manufactured (in the case of canned products, the dna obtained can be used to perform pcr in a direct way).
Advantages / Differential Points Non‑toxic reagents and safer handling: Buffers used do not contain toxic components (e.g., avoid β‑mercaptoethanol, phenol, chloroform) and can be handled without additional safety measures; no DNA‑binding resins required. Speed: Reduces extraction time from ~2 days to ~5 hours for bivalve tissues; among the fastest methods referenced by the applicants. [2332757 | PDF] Cost‑effective and accessible: Low buffer cost, no special equipment; buffers can be prepared quickly in‑house, suitable for labs with limited budgets and even non‑specialized personnel. Broad tissue and sample compatibility: Works on mantle, muscle, gonad, and gill; effective for fresh, frozen, ethanol‑preserved, and manufactured (canned) samples; in canned products, DNA can be used directly for PCR. High‑quality output and stability: Yields high‑molecular‑weight genomic DNA; DNA is stable at 4 °C (up to ~2 years) and at −20 °C (more than 3 years). Defined, simple protocol: Lysis (~20 mg tissue) at 65 °C for ~1.5 h, ammonium acetate (5 M) precipitation, isopropanol/ethanol precipitation, wash and resuspension; proteinase K step optional. Applications Food authenticity and labeling compliance for fishery and aquaculture products analyzed by food testing laboratories. Basic research applications requiring PCR‑grade genomic DNA from bivalve tissues.