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Minimal miRNA precursors
EP23382794
The most modern and highly specific RNAi tools are based on artificial sRNAs (art-sRNAs), which are computationally designed to be highly specific and bind only to the target mRNAs without interacting with other cellular RNAs. In our laboratory, we have successfully significantly reduced the size of the MIR390a precursor from Arabidopsis thaliana (AtMIR390a). Crude extracts have been obtained from plants infected with the viral vector containing the chimeric minimal precursor, and it has been possible to infect new plants and express the amiRNAs of interest by topically applying these extracts through mechanical inoculation and spraying.
ADVANTAGES - Tailor-made design adapted to the plant and the gene of interest. - The technology enables the selective inactivation of target genes, making the treatments highly specific. - Multiple treatments are not required since a single application is sufficient, resulting in reduced application costs. BUSINESS APPL Agrochemicals, breeders, and seed producers.