.jpg)
Preparation of cationic carbon points and associated gene delivery methods and systems
We present an innovative, easy, and efficient method for gene delivery that uses PEI-coated carbon dots using an electrostatic bonding process. This biofunctional approach allows the generation of optimal cationic carbon dots (CCDs) that are scalable and adaptable to various transfection needs. CCDs improve cell viability and quadruple transfection efficiency compared to current PEI polyplexus standards. This technology has proven to be especially effective in challenging transfection protocols, such as the generation of retroviral vectors by co-transfection of multiple plasmids, achieving high efficiency and functionality in the production of infective retroviral particles.
Efficient and reproducible gene delivery in human cells in culture. Solving the main challenges of gene delivery:
- High cell absorption and endosomal escape to maximize the effect of genetic material introduced into the cells.
- Low cellular toxicity, essential for safe applications.
Scalability and affordable manufacturing, suitable for biomedical and potentially clinical (gene therapy) applications.
Significant improvement in transfection efficiency (4 times higher than PEI standards). Supports complex protocols and advanced applications, such as the production of functional retroviral vectors. Use of carbon-based nanoparticles, which guarantee cell viability and reduced toxicity.
