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METHOD FOR THE DETECTION OF SCUTICOCILIATE PARASITES
Patent number:
ES2942317B2
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The present invention relates to the use of the polymerase chain reaction for detecting scuticociliates in samples, preferably scuticociliates which cause diseases in flat fish.
Countries:
Spain
Regions:
Galicia
Centers:
UNIVERSIDAD DE SANTIAGO DE COMPOSTELA, CONSEJO SUPERIOR INVESTIGACION
Other entities:
Sectors:
Chemicals
Subsectors:
TRL Level:
TRL 3 – experimental proof of concept
BRL Level:
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Applications
The inventors have developed and optimized a quantitative real-time PCR (qPCR) assay using primers and a hydrolysis probe directed to the internal transcribed spacer-2 (ITS-2) region located between the small subunit 18 S and large subunit 23S of ribosomal DNA (rDNA) that allows to quantify in a sensitive and specific way the levels of trophonts of the scuticociliate parasites P. dicentrarchi and M. avidus. This method allows the detection of very low levels of parasitic ciliates and is especially suitable for their monitoring in fish farm water.
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