NOVEL VEHICLES FOR THE TRANSFECTION OF miRNAS

Their preparation technique is very simple (one step process), mild, fast, cheap and easily scalable. In addition to avoid limitations concerning availability of materials, affordability, high cost of technologies and also intellectual property and regulatory restrictions, we can state that they are the cheapest nanoparticle system currently available for the delivery of bioactive molecules (estimated cost of 0.06 $ per mL). They are extremely versatile delivery platforms, being very easy to customize them as required in order to fulfil specific requirements such as physic-chemical properties or surface composition (main parameters affecting interaction with biological media) and in terms of association and stabilization of bioactive molecules with very different properties(i.e., low molecular drugs, antigens, growth factors, pDNA, siRNA and miRNA). Higher stability of the final products and the bioactive molecules associated to them than using conventional nanosystems (i.e., stability and clinical efficacy upon storage for months at high temperatures).•Higher biocompatibility than conventional nanoparticulate nanosystems (i.e. solid lipid nanoparticles or SLNs).