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Método para expandir el potencial de diferenciación y stemness de células pluripotentes
WO2018215662
The CNIO has developed a method for expanding stemness and differentiation potential of pluripotent cells. The inventors have found a novel and simple technique based on the transient expression of a single microRNA molecule to expand the differentiation potency of a wide range of PSCs including induced PSCs (iPSCs) as well as embryonic stem cells (ESCs).
The method of the present invention provide naïve pluripotent cells with an improved capacity to differentiate, that can be used to obtain more efficiently differentiated and mature cells proficient for regerative medicine strategies. Exposure to miR-203 improved the generation of unusual differentiated tissues. such as pancreas, bone marrow or trophoblast. The naïve pluripotent cells resultant from the method of the invention show an improved differentiation and maturation outcome, which can be observed particularly, when differentiated specifically to cardiomyocytes.