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PROCEDURE TO CORRECT THE EFFECT OF LIGHT SCATTERING IN FLAT LASER BEAM MICROSCOPE MEASUREMENTS (MACHINE-TRANSLATION BY GOOGLE TRANSLATE, NOT LEGALLY BINDING)
ES2853354
The invention describes a procedure for correcting the scattering effect of light in a flat laser beam microscope comprising: calculating, by means of Monte Carlo simulations, the transfer function of a medium for different scattering coefficients and scanning depths, creating a transfer functions database: experimentally estimate the dispersion coefficient of a sample assuming that it is uniform throughout the sample: scan the sample using the flat laser beam microscope to obtain a set of images corresponding to different sections of the sample; and deconvolve each image of the sample obtained in the scan using the transfer function of the database that corresponds to the dispersion coefficient of the sample and the scanning depth of each image, thus correcting in each image the effect of the dispersion of the light. (Machine-translation by Google Translate, not legally binding)
Método de corrección de la dispersión en microscopía de haz láser plano. - Medición 3D del grado de dispersión de la muestra, que es función de la opacidad, forma, tamaño y profundidad. - Cálculo de la función de transferencia 3D de la dispersión. - Corrección mediante posprocesado o deconvolución del grado de dispersión. Aplicación Industrial: Microscopía 3D de haz láser plano.