HEMORRHAGIC CEREBROSPINAL FLUID NEURAL STEM CELLS

1. CSF-NSCs are directly isolated from discarded liquid obtained during neuroendoscopic irrigation performed as treatment of PHH, so pose no ethical concerns. 2. CSF-NSCs are phenotypically stable through passages and adequately proliferate maintaining a stable karyotype. 3. Gene-expression profile of our cells is closer to foetal NSCs than to IPS-derived NSCs, bone marrow mesenchymal or cord blood hematopoietic stem cells. However, CSF-NSCs overexpress 112 markers that differentiate them from foetal NSCs which can be useful to isolate them. 4. Fibroblast-like cells can be isolated from these samples meaning that non-hemorragic CSF can be a new source for fibroblast/mesenchymal stem cells isolation.