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BIOTECHNOLOGICAL PRODUCTION OF D-DIBOA AND ITS CHLORINATED DERIVATES FROM ITS NITROPHENOXIDE-ACETATE PRECURSORS
WO20128117
The purpose of the invention is the biotechnological production of D-DIBOA, a compound similar to the natural herbicide DIBOA, from the precursor 2-(2'-nitrophenoxy)-ethyl acetate. This synthesis is carried out either using, as a cellular biocatalyst, the E.coli strain ?lapA?fliQ/pBAD-NfsB, designed to optimise the process, or by means of in-vitro chemical reaction catalysed by the NfsB enzyme. Using the methodologies presented in cellular biocatalysis, yields of 100 percent or concentration of up 379 percent are achieved with respect to those reported until now. Alternatively, the use of purified NfsB enzyme allows D-DIBOA to be obtained in less time. This strategy using purified NfsB enzyme also allows the production of the compounds 6-CI-D-DIBOA and 8-CI-D-DIBOA, which display greater phytotoxic activity and greater selectivity than their analogue compound D-DIBOA, with high yields.
Optimization of the production of the biological synthesis of D-DIBOA from its chemical precursor to achieve a yield of up to 100% (vs. 70% of the chemical synthesis and 60% of the biological synthesis previously reported) and an yield of up to 100% (compared to 70% of chemical synthesis and 60% of biological synthesis previously reported) and an increase of the concentration in the culture medium of up to 379% compared to increase of the concentration in the culture medium of up to 379% with respect to what has been described in the literature in biotransformation processes. biotransformation processes. This is achieved in a defined culture medium (as opposed to the use of previously used complex culture media) which will facilitate the which will facilitate the purification of the product and using a simple procedure as far as material and equipment are concerned. and equipment. Furthermore, it is carried out under gentle and environmentally friendly operating conditions. environmentally friendly operating conditions. All this makes it possible to scale up the production of D-DIBOA and its commercial exploitation.
P201831274