NUCLEIC ACID DETECTION BY HYPERBRANCHED ROLLING CIRCLE AMPLIFICATION

Patent number:

WO2024/200373

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The CNIO has developed a method for detecting the presence of a nucleic acid sequence in a sample. The inventors have found a method for detecting the presence of a nucleic acid sequence in a sample using Hyperbranched Rolling Circle Amplification technology (HRCA). The present invention also relates to a composition to carry out the method of the invention.

Countries:

Spain

Regions:

Community of Madrid

Centers:

CNIO, FUNDACION CNIO

Other entities:

Sectors:

Health

Subsectors:

Biotechnology

TRL Level:

TRL 3 – experimental proof of concept

BRL Level:

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Video Link:

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Sustainable Development Goal:

Applications

The present invention focuses on padlock design and an improved HRCA method and provides a new method for the rapid, low cost, but specific and efficient, detection of target nucleic acids. In the present invention, an HRCA assay is improved by applying different optimization strategies.

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