NUCLEIC ACID DETECTION BY HYPERBRANCHED ROLLING CIRCLE AMPLIFICATION

Patent number:

WO2024/200373

Comunidad de Madrid.svg
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The CNIO has developed a method for detecting the presence of a nucleic acid sequence in a sample. The inventors have found a method for detecting the presence of a nucleic acid sequence in a sample using Hyperbranched Rolling Circle Amplification technology (HRCA). The present invention also relates to a composition to carry out the method of the invention.

Countries:
Spain
Regions:
Community of Madrid
Centers:
CNIO, FUNDACION CNIO
Other entities:
Sectors:
Health
Subsectors:
Biotechnology
TRL Level:
TRL 3 – experimental proof of concept
BRL Level:
PDF Link:
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Video Link:
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Sustainable Development Goal:
Applications

The present invention focuses on padlock design and an improved HRCA method and provides a new method for the rapid, low cost, but specific and efficient, detection of target nucleic acids. In the present invention, an HRCA assay is improved by applying different optimization strategies.

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