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TRANSFORMED RESPONSE POTENTIOMETRIC TITRATIONS
US2017122900
The present invention relates to a method and system for obtaining an interaction property between a molecule or biomolecule or particle or bioparticle or nano- or microparticle on the one hand and a target particle on the other hand. The method comprises obtaining potentiometric titration results for a potentiometric measurement during titration of a solution with a titrant, said solution being a solution of one of a ligand of the target particle or said molecule or biomolecule or particle or bioparticle or nano- or microparticle. Said titrant comprises the other of said target particle ligand or said molecule or biomolecule or particle or bioparticle or nano- or microparticle. The method also comprises deriving based on said potentiometric titration results an interaction property between said molecule or biomolecule or particle or bioparticle or nano- or microparticle and said target particle.
Many label free methods exist to acquire quantitative information on the interaction and affinity between two different molecules or particles albeit from biological or chemical nature. Surface plasmon resonance (SPR) differential scanning calorimetry (DSC) and evanescence wave sensors are only a few examples. All of them require sophisticated and expensive instruments and expert data interpretation. Applications originate from various science fields, e.g. diagnostics and (bio)sensors, fields where both the determination of the concentration of an analyte or its affinity characteristics towards another molecule or particle are typically of interest.
More information: https://www.uantwerpen.be/en/research/info-for-companies/offer-for-companies/licence-offers/life-sciences/transf-response-potentiom-titrations/