PROBES FOR NON-INVASIVE REAL-TIME ASSESSMENT OF LIVER FUNCTION

The invention solves the incompatibility in the use of commercial fluorochromes with the few fluorescent bile acid derivatives currently available for the performance of multiparametric assays of absorption kinetics in vitro and in vivo, colocalization in microscopy, flow cytometry and image capture, focusing these applications in the field of preclinical and clinical biomedical research. The advantage of using infrared fluorescence is that it is tissue permeable, so it can be detected from outside the body, without any surgical intervention, with minimal discomfort and risk to the individual and with very little background noise. This could allow a functional evaluation of the liver in real time by means of non-invasive, highly selective and high resolution techniques, which are not available and which could be of important diagnostic and prognostic utility in a large number of pathologies that affect a high proportion of the population.